Reference Intervals for the ratio of Soluble FMS-like Tyrosine Kinase-1 and Placental Growth Factor in Pregnant and Non-Pregnant Females in Pakistani Population

Authors

  • Asma Rasheed Chughtai Institute of Pathology, Lahore, Pakistan
  • Muhammad Dilawar Khan Chughtai Institute of Pathology, Lahore, Pakistan
  • Omar Chughtai Chughtai Institute of Pathology, Lahore, Pakistan
  • Akhtar Sohail Chughtai Chughtai Institute of Pathology, Lahore, Pakistan
  • Shakeel Ashraf Chughtai Institute of Pathology, Lahore, Pakistan

DOI:

https://doi.org/10.47489/szmc.v38i3.532

Keywords:

Reference intervals, sFlt-1/PGF ratio, pre-eclampsia, rank based method

Abstract

Introduction: To derive the reference intervals of serum soluble FMS like tyrosine kinase -1/Placental growth factor ratio in pregnant and non-pregnant females.

Aims & Objectives: To derive the reference intervals of serum soluble FMS like tyrosine kinase -1/Placental growth factor ratio in pregnant and non-pregnant females.

Place and Duration of Study: A comparative Cross-sectional study conducted in department of Chemical Pathology and Immunology, Chughtai Institute of Pathology, Lahore from February 2024 till May 2024

Material & Method: Blood samples from 120 pregnant and 120 non-pregnant females from 14±1 to 27±1 weeks were taken. All participants were of reproductive age group, disease free, non-hypertensive, non-diabetic, without any inherited or autoimmune disorders and not any significant drug history. Levels of placental growth factor (PGF) and soluble FMS like tyrosine kinase -1(sFlt-1) were estimated by Elecsys cobas e601. Data was analysed by SPSS 21. Shapiro-Wilk test and Kolmogorov Smirnov test were applied. p value <0.05 revealed that data findings were non- Gaussian. Reference intervals were calculated by rank-based method.

Results: The reference intervals of serum sFlt-1/PGF ratio in non-pregnant females were 5.36 to 18.40 and in pregnant females 0.61 to 24.45 and they were determined on 2.5th and 97.5th centiles using 90% confidence interval.

Conclusion: sFlt-1/PGF ratio can act as a predictive biomarker for cardiovascular disease in non-pregnant females and for pre-eclampsia in pregnant females. Current study findings would be helpful for healthcare providers to identify high- risk groups. The identification of low-risk groups results in a shorter duration of hospitalizations with potential economic benefits.

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Published

2024-09-30

How to Cite

1.
Asma Rasheed, Muhammad Dilawar Khan, Omar Chughtai, Akhtar Sohail Chughtai, Shakeel Ashraf. Reference Intervals for the ratio of Soluble FMS-like Tyrosine Kinase-1 and Placental Growth Factor in Pregnant and Non-Pregnant Females in Pakistani Population. Proceedings S.Z.M.C [Internet]. 2024 Sep. 30 [cited 2024 Oct. 7];38(3):193-8. Available from: https://proceedings-szmc.org.pk/index.php/szmc/article/view/532