Nephroprotective Evaluation of Moringa oleifera & Beta vulgaris Leaves in Murine Methotrexate Induced Toxicity

Authors

  • Bushra Suhail Department of Pharmacology, University College of Medicine & Dentistry, Lahore
  • Shumaila Kanwel Department of Pharmacology, University College of Medicine & Dentistry, Lahore
  • Mudassara Saqib Department of Pharmacology, Shaikh Zayed Medical Complex, Lahore
  • Saadia Shahzad Alam Department of Pharmacology, Shaikh Zayed Medical Complex, Lahore

DOI:

https://doi.org/10.47489/p000s344z767mc

Keywords:

Ethanolic extract of Beta vulgaris (EEBV), Methotrexate, Ethanolic extract of Moringa oleifera (EEMO), Renal function tests (RFTs).

Abstract

Introduction: Methotrexate has been used for treatment of multiple diseases. Chronic use of this drug can result in oxidative damage resulting in nephrotoxicity. Beta vulgaris and Moringa oleifera are well known herbs for their antioxidant properties, so they can have potential nephroprotective effect against MTX induced renal damage.

Aims & Objectives: The study was designed to evaluate the therapeutic role of Moringa oleifera and Beta vulgaris leaves against MTX induced nephrotoxicity in rats.

Place and duration of study: Time duration of our research project was 6 months. It was carried out in PGMI Lahore and FPGMI, Shaikh Zayed Medical Complex, Lahore.

Material & Methods: 5 groups of 9 rats each were made. Group1 were maintained as healthy controls while Group II as diseased control Groups III, IV and V were administered daily p.o for 24 days 200mg /kg of Ethanolic Extract of Beta Vulgaris( EEBV),and 800 mg/kg Ethanolic Extract of Moringa oleifera (EEMO) and combination of extracts (100mg/kg EEBV+400mg/kg EEMO respectively. Nephrotoxicity was produced on 21st day via single i/p route injection of 20mg/kg MTX in all groups except Group 1. RFTs were performed on days 1, 21 and 24th day. Nephroprotection was determined by measuring serum urea, creatinine along with histopathological study of kidneys at study end.

Results: EEBV provided significant neproprotection in group 3 which had significantly lower levels of creatinine as compared to group 2 with p-value <0.001. The groups 4 and 5 had nonsignificant difference from group 2 with p-values 0.975 and 0.252. Further confirmation by histopathological examination of kidneys revealed group 3 to have improved status of kidney( normal kidney epithelium, normal tubules, mild congestion, mild necrosis) as compared to other groups with (distorted kidney epithelium ,dilated tubules ,moderate to severe congestion and necrosis).

Conclusion: Nephroprotective effect was shown by Beta vulgaris plant while Moringa oleifera plant extracts remained unable to show their protection against MTX induced nephrotoxicity.

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Published

2021-01-01

How to Cite

1.
Bushra Suhail, Shumaila Kanwel, Mudassara Saqib, Saadia Shahzad Alam. Nephroprotective Evaluation of Moringa oleifera & Beta vulgaris Leaves in Murine Methotrexate Induced Toxicity. Proceedings S.Z.M.C [Internet]. 2021 Jan. 1 [cited 2024 Mar. 28];34(4):30-4. Available from: https://proceedings-szmc.org.pk/index.php/szmc/article/view/6